PNC-27

Description

p53-derived peptide for selective cancer cell targeting research.

PNC-27 is a synthetic 32-amino acid peptide containing an HDM-2 binding domain derived from the p53 tumor suppressor protein, linked to a cell-penetrating leader sequence. PNC-27 selectively targets cancer cells by binding to HDM-2 (human double minute 2) protein that is aberrantly expressed on the surface membrane of transformed cells but not on normal cells. Upon binding, PNC-27 induces rapid membrane pore formation and necrosis specifically in HDM-2-overexpressing cells, a mechanism distinct from conventional apoptosis-based chemotherapy. Research by Kanovsky et al. (2001) published in Proceedings of the National Academy of Sciences demonstrated that PNC-27 killed a broad spectrum of cancer cell lines, including breast (MCF-7), pancreatic (MIA PaCa-2), and leukemia cells, while leaving normal fibroblasts and epithelial cells completely unharmed at the same concentrations. Subsequent work by Sookraj et al. in Cancer Biology & Therapy showed that PNC-27 induced rapid membranolysis within 10-15 minutes of contact with HDM-2-positive cancer cells, confirming a necrotic rather than apoptotic mechanism. Studies in ovarian cancer models demonstrated efficacy against platinum-resistant cell lines. Compared to conventional chemotherapeutic agents, PNC-27 offers a fundamentally different selectivity mechanism -- targeting a specific membrane protein (HDM-2) rather than general markers of cell division. This distinguishes it from both traditional cytotoxic agents and newer targeted therapies like tyrosine kinase inhibitors. The peptide HAMLET (human alpha-lactalbumin made lethal to tumor cells) shares the concept of selective cancer cell membrane disruption but operates through different binding targets. Store lyophilized PNC-27 at -20°C. Reconstitute with bacteriostatic water and store at 2-8°C, using within 3 weeks. PNC-27 is researched by cancer biologists, oncology researchers investigating selective antitumor agents, and cell membrane biophysicists studying peptide-membrane interactions.

This product is supplied as a lyophilized powder and is intended for laboratory and research use only. Not for human consumption. Each vial contains 10mg of research-grade material.

Research Applications

• Selective cancer cell targeting
• HDM-2 binding and membrane pore formation
• Breast and pancreatic cancer models
• Leukemia and ovarian cancer research

Specifications

Size

10mg

Purity

≥98% (HPLC)

Form

Lyophilized Powder

Molecular Formula

C188H293N53O44S

Molecular Weight

4031.7 g/mol

Storage & Handling

• Long-term storage: -20°C in a sealed, light-protected container
• Short-term storage: 2-8°C (refrigerated) for up to 30 days
• Reconstituted: Store at 2-8°C and use within 30 days
• Avoid: Repeated freeze-thaw cycles, direct sunlight, and moisture exposure
• Reconstitution: Use bacteriostatic water or sterile water for injection